Neighbor-Joining (NJ) tree inference method was originally written by Saitou and Nei, and it belongs to a class of distance-based methods used to build evolutionary trees. The NJ method takes a matrix of pairwise evolutionary distances between the given sequences to build the evolutionary tree. A phylogenetic tree for genes in marine mammals is a diagram that represents evolutionary relationships among organisms. Phylogenetic trees are hypotheses, not definitive facts. The pattern of branching in a phylogenetic tree reflects how species or other groups evolved from a series of common ancestors. It should be noted here that the raw data are provided as a distance matrix, and the initial tree is a star tree