The function of Monoclonal Antibody is depicted in this Monoclonal Antibody Production Process Diagram. Monoclonal antibodies (mAbs) are created by first exposing a mouse to an antigen and then fusing polyclonal B cells from the mouse's spleen to myeloma cells. The form-comb hybridoma cells are cultured and continue to produce antibodies against the antigen. Due to the high cost of Protein A resin, the capture step in monoclonal antibody production is a very capital-intensive operation. This Monoclonal Antibody Production Process Flow Diagram also depicts the manufacturing process, which generates mAb-producing cells by fusing myeloma cells with desired antibody-producing splenocytes (e.g., B cells).